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Friday, October 4 • 10:15am - 10:35am
AI in Screening- Application of Machine Learning Methods To Improve Hit Calling Accuracy For High Content Image Based Screens

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Application of Machine Learning Methods To Improve Hit Calling Accuracy For High Content Image Based Screens
Formation of α-synuclein inclusions is linked to pathogenesis of Parkinson’s disease. A high-content imaging screen was conducted to probe Biogen’s chemogenomic set for inhibitors of α-synuclein inclusion formation in M17 neuroblastoma cell line stably expressing α-syn 3K-GFP. Confidence in hits was hindered by high hit rate and apparent unwanted mechanism of inclusion reduction by many compounds (e.g. cytotoxicity). Improved hit-calling accuracy is much needed in this high-information content space.
 We generated a work flow to process complex, large number of image-based descriptors obtained from a high-content image-based screening campaign to identify inhibitors of α-synuclein inclusion formation. In this study, along with the user defined image analysis sequence in Columbus software, we applied a combination of unsupervised and supervised machine learning models to high-content image-based screening data of Biogen’s chemogenomic set of 3070 compounds. We demonstrated the value of this approach in confirming the hits in relation to current hit-calling and evaluate additional hits.

Speakers
avatar for Lakshmi Akella, PhD

Lakshmi Akella, PhD

Senior Scientist, Biogen
Lakshmi Akella is a computational chemist and is currently working as a Senior Scientist at Biogen in the Biotherapeutic & Medicinal Sciences Division. Prior to working at Biogen she worked at H3 Biomedicine, Broad Institute and Tripos. She holds advanced degrees in Organic Chemistry... Read More →

Chairs
avatar for Franck Madoux, PhD

Franck Madoux, PhD

Principal Scientist, Amgen
Franck Madoux heads Amgen’s Ultra-High Throughput Screening (UHTS) group where he and his team are involved in the early phases of drug discovery for small molecules via the execution of large scale biochemical, cell-based and high content assays in miniaturized formats and the... Read More →


Friday October 4, 2019 10:15am - 10:35am
Theater Ballroom (5th Fl) - Courtyard Boston Downtown 275 Tremont Street, Boston, MA 02116